Seven thermophilic
Bacillus phages were characterized with reference to their
host range, time of appearance, morphology of plaques, thermal
inactivation, stability, lipid presence and inactivation by
ultraviolet irradiation. Response surface methodology was
adapted to describe the response of growth parameters to
environmental changes. Most phages were susceptible to
temperatures above 60oC and inactivated immediately
at 103oC. Most phages were resistant to pH ranges 5
to 9 and almost all to pH 7 to 8. Both phages 46 and 80 were
highly resistance to UV exposure for 13 minutes and 20 minutes,
respectively. The presence of chloroform or 75% ethanol showed
no effect on almost all isolated phages that indicate of
possibility of the absence of lipids. The isolated phages were
slow in their growth, possibly due to the lower gross growth
efficiency.
Genetic polymorphism by RAPD-PCR and phenotypic
characteristics of isolated thermotolerant Bacillus
strains from hot springs sources
Hazem A. and Manar A.
Microbiologica 26:
249-256 (2003).
The polymerase chain reaction (PCR) based random amplified DNA (RAPD)
assay, morphological, physiological, biochemical and
antimicrobial susceptibility test methods have been evaluated
for use in the taxonomy of isolated thermotolerant Bacillus from
Jordanian hot springs, with specific reference to strain
Geobacillus stearothermophilus (ATCC 12980), Bacillus
circulans (ATCC 4513) and Bacillus sphaericus (ATCC
14577). A RAPD assay has been optimized and is able to
discriminate between numerous thermotolerant Bacillus
strains. RAPD-PCR was found to give reproducible thermotolerant
Bacillus strains classification of DNA fingerprints for
14 strains including 3 reference strains. A study of 14 isolates
and 3 reference strains, analyzing 53 phenotypic characters,
resulted in their allocation to five major clusters at 60%
similarity. Whereas at 80% similarity, twelve taxonomically
distinct groups were evident.
* Faculty:
Allied Health Sciences/ Medical Laboratory Sciences.
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