The adsorption and desorption of macromolecules on interfaces are multifaceted phenomena, which play essential roles in many technologies, such as those based on lubrication, on material adhesion and coating, and also in medicine for the development of artificial organs. The conformations assumed by the polymer molecules at the interfaces (commonly described in terms of loops, trains and tails determine the configurational entropy and enthalpy of the adsorption processes and hence the structural organisation, the thickness and the physical properties of the resulting depositions. In this Communication, we demonstrate that the scanning force microscope (SFM) in the force spectroscopy mode makes it possible to monitor, at the level of single molecules, how the extent of their cross-linking affects the adhesion energy and their conformations upon adsorption at interfaces.