Expression of an
Adhesion Related Activation Antigen on Endothelial Cells in Human Oral
M. Thornhill, S. Burgan, S.J.
Challacombe, P.R. Morgan and D.O. Haskard.
Department of Oral Medicine, Pathology and
Rhematology, UMDS, Guy’s Hospital, London, UK.
In advances in mucosal immunology eds. MacDonald T 20.5.1992 et al.
Kluwer academic publishers 615-616, 1990
At sites of inflammation
vascular endothelial cells (EC) become activated by
inflammatory cytokines and exhibit altered functions including
increased adhesiveness for circulating leukocytes. In order to
study EC activation further a monoclonal antibody 1.2 B6 was
developed by immunizing mice with human umbilical vein EC
activated for 6 hours with tumor necrosis factor a (TNE) 320 U/
ml. Antibody 1.2 B6 recognises the neutrophil adhesion related
antigen ELAM-1 (Endothelial cell- leukocyte adhesion
molecule-1). In culture ELAM-1 is not expressed no unassimilated
EC but is rapidly induced on activation of EC with TNF,
interleukin-1 (IL1) or bacterial lip polysaccharide (LPS).
Expression is maximal after 4 to re-stimulation with the same
agent but can be reticulated with another. Since most studies of
EC activation have been carried out using cultured large vein EC
there exists a need to determine whether EC in tissues behave in
a similar fashion. The aim of this study was therefore to
investigate EC activation in skin organ cultures and acute and
chronic inflammatory conditions of the oral mucosa mAb 1.2B6.
Results showed that ELAM-1 expression is modulated differently
in the oral conditions examined and in vitro. In addition ELAM-1
expression may have to exceed a certain threshold before
neutrophil adhesion and migration into the tissues can occur.